PT  - JOURNAL ARTICLE
AU  - Crystal Jaing
AU  - Shea Gardner
AU  - Kevin McLoughlin
AU  - James B. Thissen
AU  - Tom Slezak
TI  - Detection of Adventitious Viruses from Biologicals Using a Broad-Spectrum Microbial Detection Array
AID  - 10.5731/pdajpst.2011.00838
DP  - 2011 Nov 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 668--674
VI  - 65
IP  - 6
4099  - http://journal.pda.org/content/65/6/668.short
4100  - http://journal.pda.org/content/65/6/668.full
SO  - PDA J Pharm Sci Technol2011 Nov 01; 65
AB  - CONFERENCE PROCEEDING Proceedings of the PDA/FDA Adventitious Viruses in Biologics: Detection and Mitigation Strategies Workshop in Bethesda, MD, USA; December 1–3, 2010 Guest Editors: Arifa Khan (Bethesda, MD), Patricia Hughes (Bethesda, MD) and Michael Wiebe (San Francisco, CA) We designed the Lawrence Livermore Microbial Detection Array (LLMDA), which contains 388,000 DNA probes. This array can detect any sequenced viruses or bacteria within 24 h. In addition, the oligonucleotide probes were selected to enable detection of novel, divergent species with homology to sequenced organisms. We recently used this array to identify an adventitious virus from a vaccine product. We have also used this array to detect viral and bacterial infections from various human clinical samples. Broad-spectrum microbial detection microarrays are efficient and cost-effective tools to rapidly screen cell bank samples, raw materials, vaccine samples, and clinical samples to ensure drug, food, and health safety in the United States and worldwide.