PT  - JOURNAL ARTICLE
AU  - Chandrasekhar Gurramkonda
AU  - Karunasri Mupparapu
AU  - Rima Abouzeid
AU  - Yordan Kostov
AU  - Govind Rao
TI  - Fluorescence-Based Method and a Device for Rapid Detection of Microbial Contamination
AID  - 10.5731/pdajpst.2014.00951
DP  - 2014 Mar 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 164--171
VI  - 68
IP  - 2
4099  - http://journal.pda.org/content/68/2/164.short
4100  - http://journal.pda.org/content/68/2/164.full
SO  - PDA J Pharm Sci Technol2014 Mar 01; 68
AB  - A method for rapid detection of microbial detection is presented. It uses the reduction of resazurin to resorufin as an indication of the presence of viable cells. The method is highly sensitive (limit of detection 1 CFU/mL) and rapid (detection time 180 s). A portable device that could allow the detection to be performed in the field is also described. LAY ABSTRACT: Simple techniques to detect microbial contamination are needed. In particular, these need to be user-friendly and low-cost. In addition, field use capability is desirable. In this paper, we describe a device and method that has the above features.