RT Journal Article
SR Electronic
T1 Fluorescence-Based Method and a Device for Rapid Detection of Microbial Contamination
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 164
OP 171
DO 10.5731/pdajpst.2014.00951
VO 68
IS 2
A1 Chandrasekhar Gurramkonda
A1 Karunasri Mupparapu
A1 Rima Abouzeid
A1 Yordan Kostov
A1 Govind Rao
YR 2014
UL http://journal.pda.org/content/68/2/164.abstract
AB A method for rapid detection of microbial detection is presented. It uses the reduction of resazurin to resorufin as an indication of the presence of viable cells. The method is highly sensitive (limit of detection 1 CFU/mL) and rapid (detection time 180 s). A portable device that could allow the detection to be performed in the field is also described. LAY ABSTRACT: Simple techniques to detect microbial contamination are needed. In particular, these need to be user-friendly and low-cost. In addition, field use capability is desirable. In this paper, we describe a device and method that has the above features.