TABLE V

Buffer Species Sensitivity of M. orale to Low-pH Inactivationa

Test Article CompositionTreatment Time (Min)LRVb
M. orale100 mM glycine pH 3.850.5 ± 0.1
601.9 ± 0.6
M. orale100 mM glycine pH 3.550.9 ± 0.3
603.3 ± 0.4
M. orale100 mM acetate pH 3.850.7 ± 0.2
602.2 ± 0.5
M. orale100 mM acetate pH 3.551.2 ± 0.3
604.1 ± 0.5
  • a Antibody-containing eluate was titrated to pH 3.5 or 3.8, spiked with M. orale and incubated for 60 min. Samples were taken at time 0 min (sample load concentration), 5 min, and 60 min, then tittered.

  • b LRV is log reduction value.