TABLE VIII

Media and Viral Filter Retention of A. laidlawii with Virosart Filtersa

Filter TypeTest Article CompositionVolume Filtered (mL)Loading (L/m2)Load Titer (log10 CFU)bLRVc
Virosart Media Filter0.5% A. laidlawii 1X OptiCHO881756.1>5.1
921836.2>5.2
Virosart Media Filter0.1% A. laidlawii 1X OptiCHO2254505.3>4.3
2224435.6>4.6
Virosart HF Viral Filter0.5% A. laidlawii PBS + 1g/L BSA1533076.1>5.1
1503006.1>5.1
Virosart CPV Viral Filter0.1% A. laidlawii PBS + 1g/L BSA601205.2>4.2
601205.2>4.2
  • a Small-pore viral filters in general were effective barriers against mycoplasma penetration yielding at least 4.2 LRV clearance. Even at a higher load density, the second-generation filter (Virosart HF) shows substantial mycoplasma retention (5.1 Log10 CFU).

  • b CFU is colony-forming unit.

  • c LRV is log reduction value. > indicates no visible mycoplasma colonies present.