Protein A Chromatographya
| Chromatography Parameter Pattern | Flow Rate (mL/Min) | Salt Wash (Added to PBS)b | Elution pH (100 mM Glycine) | Regeneration (6 M Urea) |
|---|---|---|---|---|
| Best case | 1 | 1 M NaCl | 3.5 | Yes |
| Center point | 1 | 575 mM NaCl | 3.75 | No |
| Worst case | 1 | 150 mM NaCl | 4.0 | No |
↵a Best-case, center point, and worst-case factors previously determined as affecting the clearance of A. laidlawii were applied to M. orale and M. arginini in this study.
b PBS is phosphate-buffered saline.