Protein A Chromatographya

Chromatography Parameter PatternFlow Rate (mL/Min)Salt Wash (Added to PBS)bElution pH (100 mM Glycine)Regeneration (6 M Urea)
Best case11 M NaCl3.5Yes
Center point1575 mM NaCl3.75No
Worst case1150 mM NaCl4.0No
  • a Best-case, center point, and worst-case factors previously determined as affecting the clearance of A. laidlawii were applied to M. orale and M. arginini in this study.

  • b PBS is phosphate-buffered saline.