Peer-Reviewed Literature Reporting the Agar Growth Medium and Incubation Conditions Successful in the Recovery of Cutibacterium acnes. No Comparison or Quantification Relative or Otherwise to the Ability of Each Regimen to Recover C. acnes Is Included Here
| Medium | Temperature | Incubation Duration | Aerobic/Anaerobic | Reference |
|---|---|---|---|---|
| Robertson's cooked-meat broth | Not stated | Not stated | Anaerobic | 51 |
| Trypticase soy blood agar (TSA) plates, containing 5 mg/L hemin and 50 μg/L vitamin K | 37°C | 7 days | Anaerobic10% CO2, 10% H2, and 80% N2, or aerobically in the presence of 5% CO2. | 52 |
| Blue lactose plates under aerobic conditions | 37°C | 7 days | Aerobic | |
| Columbia sheep blood agar, Colistin-nalidixic acid (CNA) blood agar, MacConkey agar, Crowe agar (chocolate agar supplemented with bacitracin and IsoVitaleX) | Not stated | 7 days | Aerobic | 44 |
| Brucella agar (in-house sheep blood agar plates with hemin and vitamin K1), Kanamycin-vancomycin agar (laked sheep blood Brucella agar plates with kanamycin and vancomycin), Phenylethyl alcohol agar | Not stated | 7 days | Anaerobic | |
| Brain Heart Infusion agar | 37°C | 4 days | Anaerobic | 32 |
| Brain Heart Infusion agar | 37°C | 3 days | Anaerobic | 53 |
| Blood agar (Trypticase soy agar with 5% sheep blood), chocolate agar | 37°C | 28 days | Aerobic with 5% CO2 | 41 |
| Brucella agar (with blood, hemin, and vitamin K) | 37°C | 28 days | Anaerobic | |
| Chocolate agar supplemented with Polyvitex | 35°C | 21 days | Aerobic with 5% Co2 | 54 |
| CDC blood agar with a gentamicin disk (ANA CDC) | 37°C | 7 days | Aerobic with Co2 | 55 |
| Trypticase soy agar containing 5% sheep blood (Oxoid), chocolate agar (Becton Dickinson), MacConkey II agar (Becton Dickinson) | 36°C | 14 days | Aerobic | 56 |
| Schaedler agar containing vitamin K1 and 5% sheep blood (Becton Dickinson) | 36°C | 14 days | Anaerobic |