TABLE II

Representative Literature Supporting the Choice of the Current Industry Environmental and Personnel Monitoring Growth Media (Agars) and Incubation Conditions

Growth Medium	Incubation Temperature	Aerobic/Anaerobic Incubation	Incubation Duration	Purpose	Reference
Tryptone soya agar with 1% (w/w) glucose	25°C	Aerobic	5 days	Environmental monitoring for recovery of bacteria and fungi	61
Tryptone soy agar	30°C–35°C	Aerobic	5 days	Environmental monitoring for recovery of bacteria and fungi	62
Ravan medium	20°C–25°C	Aerobic	Up to 21 days	Environmental and personnel monitoring	63
Tryptic soy agar	30°C–35°C	Aerobic	3 days	Environmental monitoring, total aerobic count and mold counts^{^a}	64
Sabouraud dextrose agar	20°C–25°C	Aerobic	7 days		64
Tryptone soy agar	20°C–25°C and 30°C–35°C^{^b}	Aerobic	≥5 days and ≥2 days	Environmental monitoring bacteria and fungi	65
Sabouraud dextrose agar	20°C–25°C	Aerobic	8 days	Environmental monitoring fungi	66

↵a These conditions yielded the highest recovery for total aerobic counts and for molds, respectively; however, the authors point out that their data support the use of other conditions as acceptable.
↵b The sequence of different temperatures may be optimized depending on the environment and the process.