Table VII

Headspace Carbon Dioxide Partial Pressure in Sample Vials with Microwires at the Stopper-Seal Interface, Both Before (T₀) and After (T₁) Conducting the Container Closure Integrity (CCI) Test within ∼24 h of Sample Preparation. Values Show the Averages of Five Consecutive Measurements on Each Vial. All Vials with a Liquid Fill Had the Defect in Contact with the Liquid Just Before the CCI Test Was Performed

Nominal Wire Size [μm]	Fill	Headspace Carbon Dioxide Partial Pressure [Torr]								Number of Leaking Vials Detected^{^a}
		T₀				T₁
		Mean	StDev	Min	Max	Mean	StDev	Min	Max
41	Empty	1	1.0	1	1	78	43.3	3	105	5/5
	PBS^{^b}	1	0.1	0	1	16	35.7	0	80	1/5
	BSA^{^c} in PBS	0	0.1	0	0	0	0.1	0	0	0/5
64	Empty	1	0.0	1	1	749	126.2	532	855	5/5
	PBS	1	0.1	1	1	569	320.7	4	767	5/5
	BSA in PBS	0	0.0	0	0	606	192.9	263	713	5/5
80	Empty	1	0.2	1	1	759	426.8	1	1005	4/5^{^d}
	PBS	1	0.0	1	1	775	108.6	627	923	5/5
	BSA in PBS	0	0.1	0	0	654	49.7	592	704	5/5
NC	Empty	1	0.0	1	1	1	0.0	1	1	0/3
GPC	Empty	2	0.2	2	3	704	1.6	702	706	3/3

↵a Leakage was a change ≥ 1 torr between T₀ and T₁.
↵b PBS is phosphate-buffered saline.
↵c BSA is bovine serum albumin.
↵d One vial confirmed to not be leaking based on leak rate measurements taken after completion of the CCI protocol.