TABLE IV

Headspace Carbon Dioxide Partial Pressure in Sample Vials with Laser-Drilled Defects, Both Before (T₀) and After (T₁) conducting the CCI Test within ∼24 h of Sample Preparation. Values Show the Averages of Five Consecutive Measurements on Each Vial. All Vials with a Liquid Fill Had the Defect Located Below the liquid Level

Nominal Defect Size [μm]	Fill	Headspace Carbon Dioxide Partial Pressure [Torr]								Number of Leaking Vials Detected^{^a}
		T₀				T₁
		Mean	StDev	Min	Max	Mean	StDev	Min	Max
2	Empty	1	0.0	1	1	96	45.3	26	175	10/10
	PBS^{^b}	0	0.1	0	0	0	0.1	0	0	0/10
	BSA^{^c} in PBS	0	0.1	0	1	0	0.1	0	0	0/10
5	Empty	1	0.0	1	1	567	33.9	498	616	10/10
	PBS	0	0.1	0	0	233	169.3	21	476	10/10
	BSA in PBS	0	0.1	0	1	181	122.4	25	420	10/10
10	Empty	1	0.0	1	1	559	21.1	520	589	10/10
	PBS	0	0.1	0	0	605	181.0	261	778	10/10
	BSA in PBS	0	0.1	0	0	575	115.2	471	801	10/10
NC^{^d}	Empty	1	0.0	1	1	1	0.0	1	1	0/3
GPC^{^e}	Empty	1	0.0	1	2	709	1.0	708	710	3/3

↵a Leakage was a change ≥ 1 torr between T₀ and T₁.
↵b PBS is phosphate-buffered saline.
↵c BSA is bovine serum albumin.
↵d NC is negative control.
↵e GPC is gross positive control.