TABLE III

Summary of the Effusion Sample Set. All Vials Were Prepared with ∼760 Torr of Air in the Headspace

Storage Temperature (°C)	Control Group	Defect	Vial	Wire Size (µm)	Effective Defect Diameter (µm)^{^a}	Fill	No. of Samples
−80	Positive	Laser-Drilled	2R Glass	—	2	Empty	6
				—	2	PBS	6
				—	5	Empty	6
				—	5	PBS	6
				—	10	Empty	6
				—	10	PBS	6
	Type	Microwire	2R Glass	20	0.4	Empty	6
			2 mL Plastic	20	0.6	Empty	6
			2R Glass	41	3.2	Empty	6
			2R Glass		3.2	PBS	6
			2 mL Plastic		2.6	Empty	6
			2 mL Plastic		2.6	PBS	6
			2R Glass	64	8.3	Empty	6
			2R Glass		8.3	PBS	6
			2 mL Plastic		7.4	Empty	6
			2 mL Plastic		7.4	PBS	6
			2R Glass	80	12.9	Empty	6
			2R Glass		12.9	PBS	6
			2 mL Plastic		10.2	Empty	6
			2 mL Plastic		10.2	PBS	6
	Gross Positive	30G needle	2R Glass	—	159	Empty	6
	Negative	Unmodified	2R Glass and 2 mL Plastic	—	None	Empty	20 ea.
	Negative	Unmodified	2R Glass and 2 mL Plastic	—	None	PBS	20 ea.
−20	Gross Positive	30G needle	2R Glass	—	159	Empty	6
	Negative	Unmodified	2R Glass and 2 mL Plastic	—	None	Empty	6 ea.
	Negative	Unmodified	2R Glass and 2 mL Plastic	—	None	PBS	6 ea.

↵a Effective defect diameters listed for the laser-drilled defects are the nominal diameters reported by Lenox Laser on their calibration certificates.