The Media, Test Microorganisms, and Growth Direct® Incubation Temperature and Time at the Different Study Sites
| Site and Media | Control Microorganism | Incubation Temperature and time |
|---|---|---|
| Company 1 | B. subtilis | 30°C–35°C for 36 hours |
| TSA | P. aeruginosa | |
| R2A | C. albicans | |
| SDA | A. brasiliensis | |
| TSA + LP80 | R. pickettii | |
| S. aureus | ||
| S. epidermidis (in-house) | ||
| Penicillium species (in-house) | ||
| P. glucanolyticus (in-house) | ||
| B. cereus/thuringiensis (in-house) | ||
| Mixed culture | ||
| Company 2 | B. subtilis | 22.5°C–27.5°C for 44 hours |
| TSA + LP80 | A. brasiliensis | |
| E. coli | ||
| Company 3 | B. subtilis | 30°C–35°C for 44 hours |
| TSA + LP80 | A. brasiliensis | |
| E. coli | ||
| S. aureus | ||
| P. aeruginosa | ||
| C. albicans | ||
| Mixed culture | ||
| In-house isolates | ||
| Company 4 | B. subtilis | 28°C–32°C for 44 hours |
| TSA + LP80 | A. brasiliensis | |
| E. coli | ||
| Company 5 | EM:E. coliB. cepacia | EM:Water: |
| TSA + LP80HT | B. subtilis | 30°C–35°C for 44 hours |
| A. brasiliensis | ||
| R2A | Water: | |
| E. coli | ||
| Water: | 30°C–35°C for < 7 days | |
| B. cepacia | ||
| Company 6 | S.aureus | 30°C–35°C for 44 hours |
| TSA + LP80HT | C. albicans | |
| S. hominis | ||
| M. luteus | ||
| C. tuberculostearicum | ||
| B. subtilis | ||
| A. brasiliensis | ||
| E. coli | ||
| P. aeruginosa | ||
| Company 7 | B. subtilis | 30°C–35°C for 44 hours |
| TSA | A. brasiliensis | |
| R2A | E. coli | |
| TSA + LP80HT | ||
| Company 8 | B. subtilis | 30°C–35°C for 44 hours |
| TSA + LP80 | A. brasiliensis | |
| R2A | E. coli |