Percentage Recovery of Viable Spores after Neutralisation with Modified Letheen Broth-based Neutraliser. Percent recovery is calculated based on the number of spores inoculated, which were: B. subtilis ATCC 6633: 3.3 × 108 CFU/mL, B. subtilis 168: 5.4 × 108 CFU/mL, B. pumilis ATCC 27142: 6.5 × 107 CFU/mL. Data are represented as mean ± SEM, n = 3. P-values indicate that there were no significant differences between the percentage recovery with neutraliser only compared to any of the biocides tested.
| Spore test organisms | % Recovery of Viable Spores | ||||||
|---|---|---|---|---|---|---|---|
| Quat/Biguanide and neutraliser | Quat/Chlorine dioxide and neutraliser | 6% Hydrogen peroxide and neutraliser | Amphoteric surfactant and neutraliser | 70% v/v denatured ethanol and neutraliser | 70% IPA in WFI and neutraliser | Neutraliser only | |
| B. subtilis ATCC 6633 | 98.7 ± 1.0 | 98.2 ± 1.0 | 98.3 ± 0.3 | 97.8 ± 0.8 | 98.0 ± 0.7 | 97.4 ± 0.5 | 98.5 ± 0.5 |
| (P = 0.87) | |||||||
| B. subtilis subsp. subtilis 168 | 96.9 ± 0.9 | 96.6 ± 1.7 | 96.6 ± 2.1 | 95.6 ± 1.1 | 95.7 ± 0.3 | 95.0 ± 0.6 | 98.2 ± 0.0 |
| (P = 0.36) | |||||||
| B. pumilis ATCC 27142 | 96.3 ± 1.2 | 96.3 ± 1.3 | 96.1 ± 0.3 | 97.4 ± 1.0 | 96.3 ± 0.9 | 97.6 ± 1.4 | 97.6 ± 1.1 |
| (P = 0.85) | |||||||