Mean Percentage of B. subtilis 168, B. subtilis ATCC 6633, and B. pumilis ATCC 27142 Spores Recovered from Disinfectant Wipes, Following Wiping. Data are represented as mean ± SEM, n = 2 for B. subtilis spores and n = 3 for B. pumilis ATCC 27142 spores. Percent recovery is calculated based on the number of spores inoculated, which were B. subtilis ATCC 6633: 7.7 × 107 CFU/mL, B. subtilis 168: 7.6 × 107 CFU/mL, and B. pumilis ATCC 27142: 6.3 × 107 CFU/mL.
| Test Biocide | Average percentage of spores transferred to wipes (%) | ||
|---|---|---|---|
| B. subtilis subsp. subtilis 168 | B. subtilis ATCC 6633 | B. pumilis ATCC 27142 | |
| QUAT + Biguanide | 15.6 ± 0.12 | 21.2 ± 0.02 | 79.0 ± 0.06 |
| QUAT + Chlorine dioxide | 14.8 ± 0.11 | 24.8 ± 0.12 | 80.7 ± 0.06 |
| 6% Hydrogen peroxide | 43.5 ± 0.17 | 20.4 ± 0.04 | 79.1 ± 0.02 |
| Amphoteric surfactant | 19.7 ± 0.10 | 21.2 ± 0.00 | 80.3 ± 0.07 |
| 70% v/v denatured ethanol with deionised water | 27.1 ± 0.08 | 19.5 ± 0.04 | 41.3 ± 0.06 |
| 70% IPA with water for injection | 50.8 ± 0.11 | 50.4 ± 0.17 | 82.6 ± 0.12 |
| Sterile distilled water | 49.2 ± 0.11 | 25.7 ± 0.01 | 51.6 ± 0.03 |