Summary of Analytical Method Development (Chromatographic Configurations)
| Column | Optimized GC Oven Parameters |
|---|---|
| 1. 10% Carbowax 1540 5% KOH on 100/120 Chromosorb WHP (2.7 m × 4 mm; Supelco)—packed | 100 °C (1 min)/4 °C per min/180 °C (14 min) |
| 2. 10% Carbowax 1540 2% KOH on 100/120 Chromosorb WHP (2.7 m × 4 mm; Supelco)—packed | 100 °C (1 min)/4 °C per min/180 °C (14 min) |
| 3. 10% Carbowax 20M 2% KOH on 100/120 Chromosorb WAW (10 ft × 2 mm; Supelco)—packed | 60 °C (15 min)/6 °C per min/180 °C (25 min) |
| 4. 10% SE-2340 on 100/120 Chromosorb WHP (2.7 m × 4.7 mm; Supelco)—packed | 60 °C (15 min)/6 °C per min/180 °C (25 min) |
| 5. 3% SE-30 on 100/120 Chromosorb WHP (2.7 m × 4.7 mm; Supelco)—packed | 100 °C (1 min)/4 °C per min/180 °C (19 min) |
| 6. Stabilwax (30 m × 0.53 mm; 1 μm; Restek)—capillary | 40 °C (15 min)/4 °C per min/225 °C (8.75 min) |
| 7. DB-5 (30 m × 0.53 mm; 3 μm; J&W Scientific)—capillary | 60 °C/3 °C per min/150 °C (8 min) |
| 8. DB-624 (30 m × 0.53 mm; 3 μm; J&W Scientific)—capillary | 40 °C (5 min)/10 °C per min/100 °C/2 °C per min/130 °C/15 °C per min/160 °C (4 min) |
| 9. DB-624 (75 m × 0.53 mm; 3 μm; J&W Scientific)—capillary (final configuration) | 95 °C (10 min)/2 °C per min/120 °C/5 °C per min/200 °C (5 min) |