Operating Parameters, HPLC/UV Analysis
| Operating Parameter | Operating Value | |
|---|---|---|
| Column | Alltech (Deerfield, IL) Adsorbosphere HS, C18, 100 × 4.6 mm, 3-μm particles | |
| Column Temperature | 40 °C | |
| Mobile Phase Components | A = 0.07 M phosphoric acid, B = Acetonitrile | |
| Mobile Phase Gradient | Time | %B |
| 0.0 | 5 | |
| 12.0 | 45 | |
| 14.0 | 45 | |
| 16.0 | 5 | |
| 19.0 | 5 | |
| Mobile Phase Flow Rate | 1.0 mL/min | |
| Sample Size | 60 μL | |
| Detection, UV | 215 nm | |
| Sample Preparation | None, direct injection | |