Viral Reduction Obtained with Viruses Spiked into Process Solution and Process as Indicated
| Process Step | Viruses Used in the Viral Clearance Study | |||||
|---|---|---|---|---|---|---|
| Murine Leukemia Virus | Bovine Viral Diarrhea Virus | Adenovirus Type 2 | Pseudorabies Virus | Poliovirus | Minute Virus of Mice | |
| Viral inactivation | 2.91 | >4.92 | Not done | >5.65 | Not done | Not done |
| Chromatography number 1 | Not done | No value obtained | 2.51 | No value obtained | 3.04 | 4.57 |
| Mild heat | 2.22 | <1 | <1 | <1 | <1 | Not done |
| Nanofilter | >3.02 | >4.11 | >3.79 | >5.06 | 2.96 | 3.09 |
| Chromatography number 2 | 3.39 | 1.84 | 4.44 | 1.40 | 2.445 | Not done |
| Reduction value | 11.5 | 10.8 | 10.8 | 12.1 | 8.4 | 7.68 |