TABLE V
Additional Assays for Adventitious Virus Testing of Novel Cell Substrates
 Oncogenicity Assays (Oncogenic Viruses) |
| – Inoculation of cell lysates (from 107 cell equivalent) into newborn hamsters, mice, and rats |
| – Observation period for tumor development: 4–7 months |
 Chemical-induction Assays (Latent Viruses: Endogenous retroviruses, occult RNA viruses and oncogenic DNA Viruses) |
| – Chemical inducers: IUdR, AzaC, NaBut, TPA |
| – Sensitive and broad assays for detection of induced virus |
| ○ Conventional assays such as PERT, PCR, Infectivity, TEM |
| ○ Emerging technologies, e.g., high-throughput, massively parallel, or deep sequencing, virus microarrays, broad-range PCR assays with mass spectrometry (PLEX-ID) |