Susceptibility of Multiple CHO Cell Lines to MMV Infection. Data Courtesy of S. Liu (Genentech Unpublished Results). Strain “f52” Is the Strain Isolated from the 1994 Contamination Event. The “p” and “i” Strains Were Obtained from an Academic Laboratory (5)
| CHO Phenotype | Sensitive to | Sialic Acid Content (nmol/mg) | Virus Infectivity (log10EIA50/mL) with Stated MVM Strain | |||
|---|---|---|---|---|---|---|
| Neu5Ac | Neu5Gc | MVM (f52) | MVM (p) | MVM (i) | ||
| Type I (Process B) | All | 11.2 ± 1.7 | 0.21 ± 0.1 | 8.5 ± 0.3 | 6.0 ± 0.8 | 5.4 ± 0.9 |
| Type II | f52 > p & i | 10.7 | 0.86 ± 0.48 | 8.3 ± 1.0 | 3.4 ± 1.2 | 2.8 ± 0.8 |
| Type III | f52 ≫ p & i | 11.3 ± 1.5 | 0.24 ± 0.15 | 4.9 ± 0.5 | 2.4 | 2.5 |
| Type IV (Process A) | None | 0.3 ± 0.3 | 0.09 ± 0.06 | <0.5* | <0.5 | <0.5 |
↵* 0.5 log10EIA50/mL was the assay detection limit.