Particle Count Assessment
| DNA Genome | |||||||
|---|---|---|---|---|---|---|---|
| CTO | Titer (PFU/mL) | Total Copies (per mL) | Inf./TC (%) | Particle-Assoc. (per mL) | PC/TC (%) | Inf./PC (%) | |
| MMV | a | 2.88 × 108 | 1.95 × 1010 | 1.48 | 5.48 × 108 | 2.81 | 52.55 |
| b | 9.90 × 107 | 7.03 × 1010 | 0.14 | 3.17 × 1010 | 45.09 | 0.31 | |
| d | 1.40 × 109 | 2.53 × 1010 | 5.53 | 1.24 × 1010 | 49.01 | 11.29 | |
| e | 4.00 × 107 | 8.07 × 108 | 4.96 | 4.68 × 108 | 57.99 | 8.55 | |
| PPV | a | 1.12 × 1010 | 1.26 × 1012 | 0.89 | 1.05 × 1011 | 8.33 | 10.67 |
| b | 1.90 × 108 | 1.02 × 1012 | 0.02 | 2.98 × 1011 | 29.22 | 0.06 | |
| c | 1.89 × 108 | 9.49 × 1011 | 0.02 | 4.37 × 1010 | 4.60 | 0.43 | |
| d | 3.70 × 108 | 1.42 × 1012 | 0.26 | 2.22 × 1011 | 15.63 | 1.67 | |
| e | 8.20 × 108 | 6.52 × 1011 | 0.13 | 2.84 × 1011 | 43.56 | 0.29 | |
| PRV | a | 2.40 × 1007 | 3.17 × 1010 | 0.08 | 1.86 × 1009 | 5.87 | 1.29 |
| d | 4.37 × 1007 | 1.62 × 1010 | 0.27 | 6.86 × 1008 | 4.23 | 6.37 | |
| RNA Genome | ||||
|---|---|---|---|---|
| CTO | Titer (PFU/mL) | Particle-Assoc. (PC/mL) | Inf./PC (%) | |
| MuLV | a | 5.50 × 1007 | 5.42 × 109 | 1.02 |
| b | 8.20 × 1007 | 4.78 × 1010 | 0.17 | |
| d | 3.30 × 1007 | 1.76 × 1010 | 0.19 | |
| Reo-3 | a | 9.90 × 1007 | 1.32 × 1008 | 75.00 |
| d | 7.90 × 1007 | 2.12 × 1008 | 37.26 | |
| Summary | ||
|---|---|---|
| Virus | Inf/total | Inf/PC |
| MMV | b<a<e∼d | b<e<d<a |
| PPV | b∼c<d∼e<a | b<e<c<d<<a |
| PRV | a<d | a<d |
| MuLV | b∼d<a | |
| Reo-3 | d2<d1 | |
See Figure 1 for Derivations and Rationale.
Top: Shown are counts for viruses that contain a single-stranded (MMV, PPV) or double-stranded (PRV) DNA genome. CTO: Contract testing organization; Titer: CTO- (or source) certified titer as based on certificate of analysis; PFU: Plaque-forming units; Total copies: Total copy number per mL; Inf./TC: % infectious titer per total copy number; Particle-assoc.: Particle-associated copy number (copy number from samples treated with DNAse I prior to nucleic acid extraction); PC/TC: Ratio of particle-associated copies to total copy number; Inf./PC: % infectious titer per particle count copy number. For determining infectious particles, Inf./PC is considered the most appropriate representation. Middle: Particle count assessment for viruses that contain a single-stranded (MuLV) or double-stranded (Reo-3) RNA genome. Total genomic copy numbers cannot be accurately determined, given the low stability of free RNA. We therefore assume that the value calculated from non-DNAse I-treated samples is most representative of particle-associated copy number. Bottom: Summary of results, rank-ordered from lowest to highest, where higher values may be viewed as more desirable.