Primers and Product Sizes for PCR
| Target Microorganism | Target Gene | Primer Sequence (5′–3′) | Product Size (bp) | |
|---|---|---|---|---|
| Salmonella | Inv A | Forward | 5′ ATC GCC ACG TTC GGG CAA TTC 3′ | 275 |
| Reverse | 5′ ATC GCA CCG TCA AAG GAA CCG T 3′ | |||
| Staphylococcus aureus | m-RNA nuclease | Forward | 5′ TTC GAA AGG GCA ATA CGC AAA GA 3′ | 461 |
| Reverse | 5′ TAG CCA AGC CTT GAC GAA CTA AAG C 3′ | |||
| Escherichia coli | 16S rRNA | Forward | 5′ CCG TGT TCT CAT CCT CCC GCC TC 3′ | 559 |
| Reverse | 5′ ACG CTG ACG CTG ACC ACC GGA GA 3′ | |||
| Pseudomonas aeruginosa | oprL | Forward | 5′ CGG GCG TGC TGA TGC TCG TAT 3′ | 709 |
| Reverse | 5′ GCG CGA GGA ACG TCA GGA CAC 3′ |