TABLE I

Various Vial Stopper Combinations Using the Components Listed in This Table Were Used in Container Closure Studies after Storage at −80 °C

Material	Designation	Comments
13 mm rubber stoppers	SNB1	Stopper Non–Blow back, manufacturer 1 T_g of rubber compound: ∼ −60 °C
	SNB2	Stopper Non–Blow back, manufacturer 2 T_g of rubber compound: ∼ −65 °C
	SBB	Stopper Blow Back, manufacturer 1 T_g of rubber compound: ∼ −60 °C
2R vials (overflow capacity 4 mL)	VBB	Vial Blow Back, manufacturer 3
2R vials (overflow capacity 4 mL)	VNB	Vial Non–Blow back, manufacturer 3
13 mm caps	Plastic aluminium flip off cap	Same type of caps was used for all vial/stopper combinations