B. Operating Conditions, HPLC Method For Quantifying Group 2 Analytes1
| Operating Parameter | Parameter Value |
|---|---|
| Column | Alltech Altima C18, 150 × 4.6 mm, 5 μm particles |
| Mobile phase | 55/45 acetonitrile/0.05 M phosphate buffer, pH 2.7 |
| Mobile phase flow rate | 1.5 mL/min |
| Detection | UV at 230 nm |
| Sample size | 50 μL |
↵1 Group 2 analytes included MBA, EBA and BBA per Table I.