TABLE II

A. Operating Conditions, HPLC Method For Quantifying Group 1 Analytes1

Operating ParameterParameter Value
ColumnAlltech (Deerfield, IL) Altima C18, 150 × 4.6 mm, 5 μm particles
Mobile phase72/28 acetonitrile/0.04 M phosphoric acid
Mobile phase flow rate1 mL/min
DetectionUV at 230 nm
Sample size20 μL
  • 1 Group 1 analytes included BA, DMP, DEP, and DPP per Table I.