A. Operating Conditions, HPLC Method For Quantifying Group 1 Analytes1
Operating Parameter | Parameter Value |
---|---|
Column | Alltech (Deerfield, IL) Altima C18, 150 × 4.6 mm, 5 μm particles |
Mobile phase | 72/28 acetonitrile/0.04 M phosphoric acid |
Mobile phase flow rate | 1 mL/min |
Detection | UV at 230 nm |
Sample size | 20 μL |
↵1 Group 1 analytes included BA, DMP, DEP, and DPP per Table I.