Operating Parameter | Parameter Value | |
---|---|---|
Column | Alltech Altima C18, 150 × 4.6 mm, 5 μm particles | |
Mobile phase | Component A = 0.05 M Potassium Phosphate Monobasic, pH 3.2 | |
Component B = Acetonitrile | ||
Mobile phase gradient | Time (min) | Proportion Component A |
0.0 | 70 | |
8.0 | 20 | |
10.0 | 20 | |
14.0 | 70 | |
18.0 | 70 | |
Mobile phase flow rate | 1.0 mL/min | |
Detection | UV at 215 nm | |
Sample Size | 60 μL |
↵1 Group 3 analytes included AN, AD EAB and BAB per Table I. Group 4 analytes included MBOH, EHB and BHB per Table I.