Time in Preheated Oven (250 °C) for Vials Containing 200 μL Water
| Time in Oven | # Vials with Lamellae |
|---|---|
| 3 min | 0/20 vials |
| 4 min | 10/20 vials |
| ≥6 min | 20/20 vials |
95% alkalinity 3 cc vials (X95%) filled with 200 μL water followed by varying times in a preheated oven at 250 °C. After 3 min in the oven, vials contain residual moisture of ∼25–50 μL. 4 min is the point where vials visibly appear dry. Following oven pretreatment, vials were filled with 1 mL 20 mM glycine pH 10 and incubated at 50 °C for 24 h followed by visual inspection using APK unit.