TABLE I

HPLC Platform

HPLC ParameterValue
Column

  • Agilent Technologies Zorbax Eclipse C18

  • 100 × 3 mm i.d.

  • 3.5 μm particles

Mobile Phase

  • A: 10 mM Ammonium Acetate in Water

  • B: Acetonitrile

Flow Rate0.8 mL/min
Column Temperature50°C
Binary Gradient

  • 5–100% B in 8.4 min

  • Hold for 26.6 min

  • 5% B in 1 min

  • Hold for 3 min

Injection Volume10 μL (50 μL for SPE-LC/NMR of COC extract)
UV Wavelength205–400 nm