TABLE I

Performance Parameters

RMM
    Trade nameBactiFlow ALSBacT/ALERTAKuScreen
    CompanyAES ChemunexbioMérieuxCelsis
    PrincipleFluorescent labellingGrowth basedATP Bioluminescence
    Detection systemFlow cytometryCO2/Colour changeRLU/luminosity
    Detection typeQualitativeQualitativeQualitative
Particular
    Limit of detection1 cell per g+1 cell per sample1 cell per g+
    Sample volumepre-incubation step (1 in 100 dilution) then 50 μL samplesUp to 10 mL per 40 mL vialpre-incubation step (1 in 100 dilution) then 50 μL samples
    Capacity∼200/day∼50/day∼1000/day
25/hmodule dependant (∼240)120/h (164/run)
Time at which results are read**24 h24–72 h18 h
Ease of use
    Amount of manipulation requiredMediumEasyMedium
Cost
    Hardware*£90K£30K£67K
    Consumables per test£4£4/vial£4
  • * as of November 2010.

  • ** this was user selected and should not be confused with detection times.

  • + only after an enrichment phase.