Operating Parameters, Headspace GC-FID-MS Analysis for Volatiles
| Operating Parameter | Operating Value |
|---|---|
| Headspace Autosampler | |
| Oven Temperature | 80 °C |
| Loop Temperature | 120 °C |
| Transfer Line Temperature | 155 °C |
| Carrier gas | He at 2.4 mL/min, constant flow |
| Equilibrium Time | 120 min |
| Inject time | 0.5 min |
| Loop equilibration time | 0.30 min |
| Loop fill time | 0.30 min |
| Vial pressurization time | 0.30 min |
| Gas Chromatograph | |
| Column | J&W DB-WAXETR, 60 m × 0.32 mm I.D., 1 μm film |
| Oven Program | Start at 35 °C, hold for 7 min. Ramp at 1 °C/min to 40 °C, hold for 15 min. Ramp at 10 °C/min to 100 °C. Ramp at 25 °C/min to 240 °C, hold for 5 min. |
| MS Ionization Mode | EI+, 70 eV |
| MS Transfer Line Temp. | 240 °C |
| MS Detection Mass Range | 25 – 200 amu |
| Solvent Delay | 0 min |
| FID Temperature | 260 °C |
| FID Hydrogen Flow | 40.0 mL/min |
| FID Air Flow | 400.0 mL/min |
| FID Mode: | Constant Makeup Flow |
| FID Makeup flow: | 30.0 mL/min |
| FID Makeup gas: | Helium |
| Splitter make up gas | Helium at 4.0 psi |