Typical Operating Parameters, GC/FID and GC/MS Analyses

Operating ParameterOperating Value (Aqueous Extracts)Operating Value (Organic Extracts)
ColumnJ&W (Folsom, CA) DB-5HT, 30 m × 0.25 mm, 0.1 μm film thicknessJ&W DB-5HT, 30 m × 0.25 mm, 0.25 μm film thickness
Oven ProgramStart at 40°C, hold for 1 min; ramp at 10°C/min to 280°C, hold for 2 min; ramp at 15°C/min to 310°C, hold for 3 minStart at 50°C, hold for 1 min; ramp at 12 °C/min to 315 °C, hold for 16 min
Carrier GasHe at 1 mL/minHe at 1.2 mL/min
InjectionSplitless; 2 μLSplit (1:5); 1 μL
Injector Temperature300°C300 °C
FID Detector Temperature:350°CN/A
MS Transfer Line Temperature310°C180 °C
MS Detection Details70 eV (+EI), mass range of 33–650 amu (5.0 min or 7.5 min solvent delay used for un-derivatized or derivatized samples)70 eV (+EI), mass range of 33–650 amu (3.0 min solvent delay)
Instrumentation Used
  1. Agilent (Santa Clara, CA) 6890 GC/7683 autosampler/5973 MSD Simultaneous FID and MS via flow splitter (MSD Productivity Chemstation software)

  2. Agilent 6890 GC/CTC Analytics CombiPal aitosampler/Waters (Millford, MA) GCT Premier TOF-MS (Waters MassLynx 4.1 software)

Agilent 6890 GC/7683 autosampler/973 or XL5975C MSD (MSD Productivity Chemstation software)