Equivalence Data Biofilm Consortiums
| Suspending Medium | 200 mL 1.5:1 BSS: Fluid D | ||
|---|---|---|---|
| Estimated cfu/vial | 10–50 cfu | 100–500 cfu | 1000–5000 cfu |
| Estimated cfu/200 mL flask | 50–250 cfu | 500–2500 cfu | 5000–25,000 cfu |
| Test Organism Consortium | Percent Recovery1 | Percent Recovery | Percent Recovery |
| Consortium 1—replicate 1 | (47/39) 120% | (460/400) 115% | (4065/4730) 86% |
| Consortium 1—replicate 2 | (56/63) 89% | (378/460) 82% | (4509/4165) 108% |
| Consortium 1—replicate 3 | (49/63) 78% | (246/300) 82% | (2925/3310) 88% |
| Consortium 2—replicate 1 | (161/190) 85% | (1796/1180) 152% | (19,451/12,633) 154% |
| Consortium 2—replicate 2 | (160/180) 89% | (1218/1260) 97% | (12,841/11,167) 115% |
| Consortium 2— replicate 3 | (63/59) 107% | (468/480) 98% | (4927/5140) 96% |
| Consortium 2—replicate 4 | (57/51) 112% | (665/400) 166% | (5494/4534) 121% |
| Consortium 2—replicate 5 | (90/73) 123% | (795/580) 137% | (7398/6700) 110% |
| Consortium 3—replicate 1 | (8/7) 114% | (18/16) 113% | (246/220) 112% |
| Consortium 3—replicate 2 | (101/60) 168% | (744/440) 170% | (7992/5700) 140% |
| Consortium 3—replicate 3 | (129/104) 124% | (1683/1100) 153% | (15,095/10,800) 140% |
| Consortium 3—replicate 4 | (176/180) 98% | (1499/1250) 119% | (16,557/13,567) 122% |
↵1 Percent Recovery = (counts ScanRDI per 100 mL/counts reference method per 100 mL) × 100; corrected for dilution.