Overview of Study Design and Extraction Parameters
| Typical Sample Preparation | 5 g test article/200 mL extraction solvent | 1 g/20 mL vial | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Extraction Method | Soxhlet | Reflux | Sonication | Sealed Vessel | Headspace | |||||||
| Extraction Solvent | IPA | Hexane | IPA | Hexane | IPA/W | pH 2.5 | pH 9.5 | pH 2.5 | pH 9.5 | IPA/W | ||
| Test Article | PVC | X | X | X | X | X | X | X | X | X | X | X |
| Rubber | X | X | X | X | — | X | X | X | X | X | X | |
| LDPE | X | X | X | X | — | X | X | X | X | X | X | |
| PC | X | X | X | X | X | X | X | X | X | X | X | |
| COC | X | X | X | X | — | X | X | X | X | X | X | |
| Extraction Parameters | 16–24 h | 2–3 h | 2 h | 0 °C 2 h | Autoclave at 121 °C 1 h | 55 °C 3 days | 80 °C 2 h | |||||
| Analytical Method | GC/FID or GC/MS | X (1) | X (1) | X (1) | X (1) | X (1,2) | X (1,2) | X (1,2) | X (1,2) | X (1,2) | X (1,2) | — |
| LC/UV/MS | X (1) | X (1) | X (1) | X (1) | X | X | X | X | X | X | — | |
| ICP/MS | — | — | — | — | — | X | X | X | X | — | — | |
| Headspace GC/MS | — | — | — | — | — | — | — | — | — | — | X | |
Notes: An “X” denotes a material/solvent/analytical method combination for which testing was performed, a “—“ denotes a combination not tested.
IPA = isopropanol, IPA/W = 50/50 isopropanol/water
1. Sample processing included concentration (see text).
2. Sample processing included solvent-switching for improved compatibility between solvent and analytical method as well as analysis of TMS-derivatized and underivatized extracts (see text).