Amgen's DOE Screening Study Evaluating the Effect of Static Hold on MMV Clearance by Viresolve Pro Filters
| Buffer Condition | pH | mAb Conc (g/L) | Pressure (psi) | LRV Results by Infectivity Assay | |
|---|---|---|---|---|---|
| Before hold | After 20 h hold | ||||
| 25 mM Tris | 7.5 | 5 | 50 | ≥5.41 | ≥5.35 |
| 50 mM NaAcetate, 500 mM NaCl | 4.5 | 5 | 50 | ≥5.84 (inline) | ≥5.78 |
| 25 mM Tris, 500 mM NaCl | 7.5 | 5 | 10 | ≥6.10 | ≥6.04 |
| 50 mM NaAcetate | 4.5 | 40 | 10 | ≥5.75 | ≥5.68 |
| 25 mM Tris, 500 mM NaCl | 7.5 | 40 | 50 | 5.20 | 5.15 |