Abbott Study Comparing Membrane Filtration Devices with Two Different Antibodies and Three Buffer Compositions. Load Comp, Load Composition; nt, Not Tested; Na Phos., Sodium Phosphate Buffer; NaCl, Sodium Chloride Buffer
| Molecule | Load pH | Load Cond. (ms/cm) | Load Composition | Medium | Load Amount | Total Virus in Load (log) | Viral Clearance (LRF ) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| XMuLV | MMV | Reo 3 | PRV | XMuLV | MMV | Reo 3 | PRV | ||||||
| mAb A | 8.0 | 5.0 | Trolamine, Acetate, Phosphate | Q Sepharose FF Resin | 80 g/L | nt | 7.8 | nt | ≥5.6 | ||||
| Pall Mustang Q Membrane | 2.7 g/mL | 7.4 | 7.2 | ≥5.1 | ≥5.4 | ||||||||
| 5.9 g/mL | ≥5.0 | 5.9 | |||||||||||
| 9.3 g/mL | ≥5.1 | 5.1 | |||||||||||
| Sartorius Sartobind Q Membrane | 3.0 g/mL | 6.4 | 7.9 | ≥3.7 | 4.6 | ||||||||
| 6.1 g/mL | ≥3.7 | 4.8 | |||||||||||
| 10.2 g/mL | 4.11 | 5.1 | |||||||||||
| Cuno VR-07 Filter | 3.0 g/cm2 | 8.0 | 8.0 | ≥4.3 | 3.87 | ||||||||
| 3.0 g/cm2 | ≥4.3 | 2.93 | |||||||||||
| 3.0 g/cm2 | ≥4.3 | 2.98 | |||||||||||
| mAb B | 8.0 | 5.5 | Trolamine, Acetate, Phosphate | Pall Mustang Q | 3 g/mL | 7.0 | 7.6 | ≥4.6 | ≥4.3 | ||||
| 6 g/mL | ≥4.6 | ≥4.3 | |||||||||||
| 10 g/mL | ≥4.6 | 3.0 | |||||||||||
| 8.0 | 3 g/mL | 7.3 | 7.8 | ≥4.9 | 3.6 | ||||||||
| 6 g/mL | ≥4.9 | 3.6 | |||||||||||
| 10 g/mL | ≥4.9 | 3.4 | |||||||||||
| mAb C | 8.0 | 5.0 | Trolamine, Acetate, Phosphate | Sartorius Sartobind Q | 5.0 g/mL | 7.8 | 9.7 | ≥5.2 | 5 | ||||
| mAb D | 6.2 | 4.5 | Tris, Acetate, Phosphate | Sartorius Sartobind Q | 5.0 g/mL | 8.0 | 10.1 | ≥5.4 | 4.8 | ||||
| mAb E | 8.0 | 4.0 | Trolamine, Acetate, Phosphate | Pall Mustang Q | 0.7 g/mL | 6.9 | 8.2 | ≥4.6 | ≥4.4 | ||||
| 1.3 g/mL | ≥4.6 | 4.2 | |||||||||||
| mAb F | 8.0 | 5.0 | Trolamine, Acetate, Phosphate | Pall Mustang Q | 1.5 g/mL | 7.09 | 8.75 | ≥6.2 | 3.4 | ||||
| 2.5 g/mL | ≥6.4 | 3.3 | |||||||||||
| mAb g | 7.2 | < 15 | Sodium Phosphate, Sodium Chloride | Pall Mustang Q | 0.6 g/mL | 7.7 | 8.3 | 8.2 | 8.3 | 2.9 | 0.4 | 3.9 | 4.7 |