Characteristics of Amgen Molecules That Achieve >4 LRVs of xMuLV Clearance at pH 5.0 (Table IV in Reference 2). There was no significant impact on xMuLV removal of variety of HMW/CHOP levels.
| Molecule | pI | Product Loading (g/L Resin) | HMW (%) in Load | HCP (ng/mg) in Load |
|---|---|---|---|---|
| mAb 1 | 6.8 | 45 | 0.5 | 10 |
| mAb 2 | 9.0 | 25 | 0.8 | 300 |
| mAb 3 | 8.2 | 35 | 1.5 | 1200 |
| mAb 4 | 7.2 | 40 | 1.9 | 200 |
| mAb 5 | 7.5 | 40 | 3.0 | 130 |
| mAb 6 | 8.2 | 40 | 3.0 | 1850 |
| mAb 7 | 6.9 | 30 | 1.4 | 500 |
| mAb 8 | 8.9 | 40 | 1.0 | 1050 |
| mAb 9 | 7.2 | 40 | 1.6 | 170 |
| mAb 10 | 7.0 | 60 | 1.9 | 500 |
| mAb 11 | 8.2 | 50 | 0.4 | 40 |
| mAb 13 | 8.9 | 60 | 3.0 | 60 |
| mAb 15 | 8.7 | 60 | 1.4 | 1750 |
| mAb 16 | 7.4 | 70 | 4.1 | 1150 |
| mAb 17 | 8.8 | 60 | 2.0 | 80 |