Subtle Shifts in the pH of a Janssen Monoclonal Antibody Process Pool at Various Protein Concentrations after Addition of Spiking Virus Media. Data Courtesy of Pedro Alfonso and Tiffany Cheung
| Test Condition | pH Measurement at Various mAb Concentrations | |||
|---|---|---|---|---|
| 4 mg/mL | 9 mg/mL | 11 mg/mL | 20 mg/mL | |
| Start pH of DPC eluate | 3.81 | 3.86 | 3.69 | 3.80 |
| pH @ Room Temp after media addition | 3.96 | 3.98 | 3.80 | 3.86 |
| pH @ 19 °C after media addition | N/A | N/A | 3.83 | N/A |
| pH @ 18 °C after media addition | 4.09 | 4.03 | 3.87 | 3.98 |