Summary of Amgen DOE Screening Study of MMV removal by Viresolve Pro
| Buffer Condition | pH | mAb Concentration (g/L) | Pressure (psi) | Loading (L/m2) | % Flow Decay | LRV Results by Infectivity Assay | |
|---|---|---|---|---|---|---|---|
| Run 1 | Run 2 | ||||||
| 25 mM Tris | 7.5 | 5 | 50 | 500 | 5% | ≥5.30 | ≥5.41 |
| 50 mM NaAcetate, 500 mM NaCl | 4.5 | 5 | 50 | 125 | >95% | ≥5.96 | 5.31 |
| 500 (inline) | 40% | ≥5.99 | ≥5.84 | ||||
| 25 mM Tris, 500 mM NaCl | 7.5 | 5 | 10 | 500 | 50% | ≥6.09 | ≥6.10 |
| 50 mM NaAcetate | 4.5 | 40 | 10 | 500 | 50% | ≥5.77 | ≥5.75 |
| 25 mM Tris, 500 mM NaCl | 7.5 | 40 | 50 | 300 | 90% | 5.35 | 5.20 |
| 500 (inline) | 60% | ≥5.79 | − | ||||