Effect of mAb Loading on xMuLV Elution at pH 5.0 in an Amgen Study (Published as Table III in Reference 2). Both virus and mAb bind to Fractogel SO3− at pH 5.0×. MuLV elution is not significantly affected by mAb loading.
| Fraction (mM sodium) | Total xMuLV Detected (Logs)a | ||
|---|---|---|---|
| No mAb | mAb (40 g/L resin) | mAb (80 gIL resin) | |
| 325–350 | ≤2.2 | ≤2.2 | ≤4.2b |
| 350–375 | ≤2.2 | ≤2.2 | ≤4.3b |
| 375–400 | 3.3 | 2.9 | 4.1 |
| 400–425 | 3.6 | 3.5 | 4.2 |
| 425–500 | 4.6 | 4.6 | 5.8 |
| 500–800 | 6.0 | 6.5 | 7.0 |