Viruses Intended for Inclusion in Virus Clearance Demonstration for Vaccine Candidate Produced in Insect Cell/Baculovirus System
| Virus | Virus Type | Size | Rationale for Inclusion |
|---|---|---|---|
| Baculovirus | Enveloped | 250–300 nm | High titer (∼109 vp/mL) in cell culture harvest |
| XMuLV | Enveloped | 80–130 nm | Model retrovirus; reverse transcriptase activity is present in some insect cell lines |
| PPV | Non-enveloped | 18–26 nm | Non-enveloped virus. PPV was selected over MMV for this vaccine candidate because the process streams presented significant toxicity issues for the indicator cells used for the MMV infectivity assay |