An Evaluation of the Impact of Vendor Resin Expiry on Viral Clearance during the Lifetime of Protein A and Anion-Exchange Chromatography Resins. Data provided by Brett Hanna, Rebecca Smith, Christopher Rode, and Michael Spade.
| Unexpired Resin | Expired Resin | |||||
|---|---|---|---|---|---|---|
| Reused Resin (LRV) | Control Resin (LRV) | Normalized LRV (%) | Reused Resin (LRV) | Control Resin (LRV) | Normalized LRV (%) | |
| Protein A chromatography, MAb-2 | ||||||
| Ecotropic Recombinant Virus of mice (ERV) | 6.6 | 6.5 | 102 | 6.6 | 6.5 | 102 |
| Polio | 4.3 | 4.3 | 100 | 4.4 | 4.3 | 102 |
| MVM | 4.4 | 3.7 | 119 | 4.2 | 3.7 | 114 |
| Yield decay | No | 46% (P < 0.0001) | ||||
| DBC decay | 58% (P = 0.03) | 68% (P = 0.01) | ||||
| Protein A chromatography, MAb-3 | ||||||
| ERV | 4.4 | 4.4 | 100 | 4.6 | 4.4 | 105 |
| Polio | 4.5 | 3.7 | 122 | 5.0 | 3.7 | 135 |
| MVM | 2.7 | 4.0 | 68 | 4.3 | 4.0 | 108 |
| Yield decay | 14% (P < 0.0001) | 13% (P < 0.0001) | ||||
| DBC decay | 21% (P = 0.01) | 18% (P = 0.004) | ||||
| Anion-exchange chromatography, MAb-2 | ||||||
| ERV | 5.5 | 5.6 | 98 | 4.7 | 5.6 | 84 |
| Reovirus | 5.8 | 5.8 | 100 | 6.5 | 5.8 | 112 |
| MVM | 4.6 | 4.4 | 105 | 4.6 | 4.4 | 105 |
| Yield decay | No | No | ||||
| Ion capacity decay | No | No | ||||
| Anion-exchange chromatography, MAb-3 | ||||||
| ERV | 5.6 | 6.6 | 85 | 5.6 | 6.6 | 85 |
| Reovirus | 6.3 | 6.3 | 100 | 6.2 | 6.3 | 98 |
| MVM | 6.9 | 6.3 | 110 | 6.6 | 6.3 | 105 |
| Yield decay | No | No | ||||
| Ion capacity decay | No | No | ||||