Inactivation of XMuLV Using a Wash Step during Protein A Chromatography
| Inactivating Agent | Static Hold (minutes) | Overall LRV (TCID50) |
|---|---|---|
| Control (Equilibration buffer) | None | 1.35 |
| Salt A | 30 | ≥6.34 |
| 3M Urea/10% IPA | None | 5.65 |
| 0.1% Triton X-100 | None | 5.84 |
| 0.1% Triton X-100 | 30 | ≥6.80 |