Inactivation of XMULV and Binding Activity of an Fc Fusion Protein in HCCF after Incubation with Zwitterionic Detergents for 120 min at ∼20 °C
| Detergent Concentration at 10× CMC (%, w/v) | LRV (120 min) | Percent Relative Binding Activity | |
|---|---|---|---|
| SB-12 | 1.3% | ≥4.2 | 97% |
| SB-14 | 0.14% | ≥4.7 | 92% |
| SB-16 | 0.024% | ≥4.2 | 92% |
| LDAO | 0.46% | ≥4.0 | 93% |
Virus inactivation was measured using median tissue culture infectivity dose (TCID50) infectivity assay. Binding activity was measured after purification of the Fc fusion protein by protein A affinity chromatography and is expressed relative to an untreated reference sample of the fusion protein.