Operating Parameters for the LC/MS/MS Method for Measuring DEHP in the Albumin and Polysorbate 80 Test Extracts
| Operating Parameter | Operational Condition | |
|---|---|---|
| Column | Phenomenex Kinetex C18, 50 × 2.1 mm, 2.6 μ particle size | |
| Column Temperature | 40 °C | |
| Injection Size | 2 μL | |
| Mobile Phase A | 10 mM Ammonium formate + 0.1% formic acid in water | |
| Mobile Phase B | 0.1% Formic acid in acetonitrile | |
| Mobile Phase Gradient | Time (min) | % Mobile Phase B |
| 0 | 82 | |
| 1.85 | 82 | |
| 1.90 | 98 | |
| 2.50 | 98 | |
| 2.55 | 82 | |
| 3.00 | 82 | |
| Mobile Phase Flow Rate | 0.6 mL/min | |
| MS Operating Mode | APCI, + ion | |
| MS/MS Scan Segments | For DEHP, m/z = 391.3/149 amu; for DOP D-4 (internal standard), m/z = 395.3/153 amu | |