Fluorescence Measurements* Using Optimized Instrument Parameters
| Measurement Number | Methylene Blue in Syringe | Rhodamine in Syringe | |||
|---|---|---|---|---|---|
| Concentration 0.2 ug/mL | Concentration 0.2 ug/mL | Concentration 0.05 ug/mL | Concentration 0.005 ug/mL | Concentration 0.005 ug/mL | |
| 1 Syringe, 10 Readings, Re-positioning the Syringe for Each Reading | 10 Syringes, 1 Reading for Each | 1 Syringe, 10 Readings, Re-positioning the Syringe for Each Reading | 1 Syringe, 10 Readings, Re-positioning the Syringe for Each Reading | 10 Syringes, 1 Reading for Each | |
| 1 | 89.6 | 94.8 | 964.2 | 105.5 | 144.6 |
| 2 | 87.0 | 95.5 | 970.0 | 107.1 | 138.8 |
| 3 | 88.5 | 89.2 | 964.2 | 106.6 | 138.8 |
| 4 | 88.3 | 107.4 | 964.2 | 105.7 | 155.7 |
| 5 | 90.5 | 115.5 | 986.1 | 107.7 | 148.8 |
| 6 | 88.0 | 79.1 | 964.8 | 105.4 | 141.9 |
| 7 | 89.2 | 106.5 | 959.1 | 105.8 | 149.0 |
| 8 | 87.0 | 97.5 | 964.2 | 106.5 | 120.8 |
| 9 | 86.8 | 114.3 | 957.0 | 109.0 | 146.6 |
| 10 | 88.0 | 108.5 | 958.3 | 105.8 | 149.6 |
| Mean | 88.3 | 100.8 | 965.2 | 106.5 | 143.5 |
| SD | 1.2 | 11.6 | 8.3 | 1.1 | 9.5 |
| % RSD | 1.4 | 11.5 | 0.9 | 1.1 | 6.6 |
↵* Instrument parameters: PMT voltage: 800 V; excitation slit: 10 R; emission slit: 10; with syringe sample holder.