Formula of YNA with metallic salt solution
| Nutrient broth (Difco) | 10 g |
| Yeast extract | 3 g |
| Agar | 20 g |
| Distilled water | 980 mL |
| Metallic salt solution A* | 10 mL |
| Metallic salt solution** | 10 mL |
| *Metallic salt solution A | |
| MnCl2·4 H2O | 2.5 g |
| MgSO4·7 H2O | 25 g |
| FeSO4·7 H2O | 30 mg |
| 0.01 mol HCl | 1000 mL |
| **Metallic salt solution | |
| CaCl2·2 H2O | 15 g |
| 0.01 mol HCl | 1000 mL |