Description of Primary Challenge Organisms
| Microorganism | Description | Optimum Growth Temperature (°C) | Appearance in TSB |
|---|---|---|---|
| Pseudomonas aeruginosa | Gram-negative, rod-shaped, obligate aerobe | 37 | Turbid growth |
| Staphylococcus aureus | Gram-positive cocci, facultative anaerobe | 35–37 | Turbid growth |
| Bacillus subtilis subsp. spizizenii | Gram-positive, spore-forming rod, obligate aerobe | 30–37 | Flocculent/surface growth |
| Candida albicans | Yeast, facultative anaerobe | 25–30 | Flocculent/surface growth |
| Aspergillus brasiliensis | Fungus, obligate aerobe | 24–30 | Flocculent/surface growth |