Viral Clearance on New and Cycled (>108) Protein A Chromatography Media
| Step | Log10 Viral Titer (by qPCR) | Log10 PFU (by plaque assay) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X-MuLV | PRV | PPV | Reo-3 | |||||||||
| Run 1 | Run 2 | Cycled | Run 1 | Run 2 | Cycled | Run 1 | Run 2 | Cycled | Run 1 | Run 2 | Cycled | |
| Load | 9.13 | 9.11 | 8.83 | 8.67 | 8.64 | 9.19 | 10.34 | 10.52 | 9.90 | 9.22 | 9.38 | 8.69 |
| Nonbound/Wash 1 | 9.11 | 8.94 | 8.93 | 8.47 | 8.59 | 9.19 | 10.20 | 10.40 | 9.59 | 9.36 | 9.29 | 8.94 |
| Wash 2/Pre-Elution | 4.40 | 3.99 | 4.72 | 5.27 | 5.29 | 5.81 | 7.46 | 7.47 | 7.26 | 5.43 | 5.33 | 4.87 |
| Eluate | 5.83 | 5.68 | 4.25 | 5.59 | 5.72 | 4.51 | 7.87 | 7.90 | 6.89 | 6.73 | 6.66 | 5.82 |
| Post-Elution/Cleaning | 7.17 | 7.14 | 6.45 | 6.20 | 6.07 | 6.27 | 6.17 | 6.25 | 8.02 | <3.60 | <3.58 | <3.78 |
| Regeneration | 4.58 | 4.94 | 5.48 | 5.96 | 6.41 | 7.36 | <4.27 | 4.58 | <4.27 | <3.28 | <3.26 | <3.75 |
| Storage | 4.67 | 4.38 | 3.27 | 3.73 | 3.92 | 4.82 | 5.99 | 5.97 | <2.35 | 3.51 | 3.34 | <2.36 |
| LRV (Load–Eluate) | 3.30 | 3.43 | 4.58 | 3.08 | 2.92 | 4.68 | 2.47 | 2.62 | 3.01 | 2.49 | 2.72 | 2.87 |