Typical Operating Parameters, LC/UV/MS Analyses
| Operating Parameter | Operating value | |
|---|---|---|
| Column | Agilent Zorbax Eclipse Plus C18, 100 × 3.0 mm, 3.5 μm particles | |
| Column Temperature | 50 °C | |
| Mobile Phase Components | A = 10 mM ammonium acetate, B = acetonitrile | |
| Mobile Phase Gradient | Time | % B |
| 0.0 | 5 | |
| 8.4 | 100 | |
| 21.0 | 100 | |
| 24.0 | 5 | |
| 26.0 | 5 | |
| Mobile Phase Flow Rate | 0.8 mL/min | |
| Injection Volume | 10 μL | |
| Detection, UV | 205–400 nm; step = 2 nm | |
| Detection, MS | API-ES or AP-CI, positive ion and negative ion (targeted m/z for each targeted analyte) | |
| Instrumentation Used | Agilent 1200 LC (vacuum degasser, binary pump, heated column compartment)/ Model G1315A diode array detector/6120 mass selective detector (MSD). Agilent Chemstation rev B.04.03-SP2 data system | |