Operating Parameters, LC/MS/UV Analysis of the Static Extracts
| Operating Parameter | Operating Value | |
|---|---|---|
| Column | Agilent ZORBAX Eclipse Plus C18, 2.1 × 100 mm, 3.5 um particle size | |
| Column Temperature | 40°C | |
| Mobile Phase Components | A = 10 mM Ammonium acetate, B = methanol | |
| Mobile Phase Gradient | Time (min) | % B |
| 0.0 | 5 | |
| 1.0 | 5 | |
| 20.0 | 99 | |
| 30.0 | 99 | |
| 30.1 | 5 | |
| 35.0 | 5 | |
| Mobile Phase Flow Rate | 0.5 mL/min | |
| Sample Size | 60 μL | |
| Detection, UV | 210, 230, 250, 280 nm | |
| Detection, MS | API-ES, positive ion and negative ion (range 80–1200) | |